Residual thiosulfate determination in omeprazole by liquid chromatography Mehmet Altun, Duran Karakaş and Gökhan Bilsel J. Chem. Metrol. (2016), 10:2 ; 27-31 Department of Chemistry, Istanbul University, Avcılar, P.O. Box 34320, Istanbul, Türkiye Department of Environmental Engineering, Faculty of Engineering and Architecture, Abant Izzet Baysal University, P.O. Box 14280 Bolu, Türkiye Department of Chemistry, TUBITAK UME (National Institute of Metrology), P.O. Box 41470, Gebze-Kocaeli, Türkiye TUBITAK, UME, P.Box:54 41470 Gebze-Kocaeli, Türkiye Abstract: An HPLC-UV method for the determination of thiosulfate in aqueous media was modified and validated in this study to quantify the amount of residual thiosulfate in omeprazole samples. Absorption of thiosulfate ion was monitored at 215 nm in alcoholic tetrabutylammonium hydroxide solution as a mobile phase, in which the pH was adjusted to 6.20 by using dilute hydrochloric acid. A method detection limit of 0.08 mg/L was achieved for thiosulfate ion in 1000 mg/L omeprazole solution. The precision, accuracy, and the expanded uncertainty of the method were 2.7 %, 92.4-102.6 %, and 2.2 % (at 95 % confidence level (k = 2) assuming 1.0 mg/L thiosulfate in the sample solution), respectively. Keywords: HPLC-UV; omeprazole; residual thiosulfate; method validation. © 2016 ACG Publications. All rights reserved. |
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