Journal of Chemical Metrology

Year: 2018 Volume:  12 Issue: x

 

  ORIGINAL ARTICLE

1.

Rapid and reliable 25-OH vitamin D2 and 25-OH vitamin D3 measurements by multitasker LC-MS/MS

Ahmet C Gören, Gözde Şahin, İsmail Gümilcineli and Burcu Binici

TÜBİTAK UME (National Metrology Institute), Organic Chemistry Laboratories, 41470, Gebze-Kocaeli, Türkiye

Zivak Technologies, Gürsel Mh. Eski Beşiktaş Cad. No: 44/1-2 34400, Kağıthane, İstanbul, Türkiye

 

Abstract:The 25-OH Vitamin D2 and 25-OH Vitamin D3 measurement method was validated using a Zivak Multitasker Fully Automated Sample Preparation and Injection System coupled to a MS detector. The current method incorporates all sample preparations, SPE clean up procedures, LC and MS facilities into an all in one system. This system can report patient data within 6 minutes including the SPE and Sample preparation and mass spectrometry analysis time. The correlation coefficient of the developed method was greater than 0.99 for measurands, 25-OH-vitamin Ds, in the calibration range. The measurement uncertainty of the measurements were evaluated and found as 7.7 % and 7.6 % for 25-OH vitamin D2 and 25-OH-vitamin D3, respectively.

 Keywords: 25-OH Vitamin D2; 25-OH Vitamin D3; MS/MS; ZİVAK multitasker; method validation; uncertainty. © 2018 ACG Publications. All rights reserved.