11.

Cytotoxic Activity of β -Caryophyllene Oxide Isolated from Jeju Guava (Psidium cattleianum Sabine) Leaf

Neung Jae Jun, Ashik Mosaddik, Jeong Yong Moon, Ki-Chang Jang, Dong-Sun Lee, Kwang Seok Ahn and Somi K. Cho 

Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea

Department of Functional Crop, National Institute of Crop Sciencee, RDA, Milyang 627-803, Republic of Korea

The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University, Jeju 690-756,Republic of Korea

Subtropical Horticulture Research Institute, Jeju National University, Jeju 690-756, Republic of Korea

Department of Oriental Pathology, College of Oriental Medicine, Kyung Hee University, Hoegi-Dong Dongdaemun-Gu, Seoul 130-701, Republic of Korea

Abstract: β -caryophyllene oxide was isolated from the leaf of the Jeju guava (Psidium cattleianum Sabine) for the first time in this study. The chemical structure was confirmed with the help of various spectroscopic analyses, including NMR data, by comparison to that of the published data. An MTT assay was conducted against several cancer cell lines t o resolve an ongoing controversy regarding the cytotoxic effects of β-caryophyllene oxide. β-caryophyllene oxide evidenced potent cytotoxic activity against HepG2, AGS, HeLa, SNU-1, and SNU-16 cells, with IC 50 values of 3.95, 12.6, 13.55, 16.79, and 27.39 μM, respectively. The results also showed that β-caryophyllene oxide evidenced cytotoxicity in both a dose-dependent and time-dependent manner.

Keywords: β-caryophyllene oxide; cytotoxicity; Jeju guava; Psidium cattleianum