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Bioactive Compounds of Endemic Species Sideritis raeseri subsp. raeseri Grown in National Park Galičica

Nebojša Menković, Dejan Gođevac, Katarina Šavikin, Gordana Zdunić, Slobodan Milosavljević, Andon Bojadži and Oliver Avramoski

Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia

Institute for Chemistry, Technology and Metallurgy,University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia

Serbian Academy of Science and Art, Knez Mihailova 35, 11000 Belgrade, Serbia

Public Institution Galicica National Park, Galičica bb, 6000 Ohrid, Former Yugoslav Republic of Macedonia

Abstract: Collection of Sideritis raeseri subsp. raeseri has a long tradition in local communities in the Ohrid-Prespa region. The aim of the present study was the analysis of bioactive compounds especially those with anti-inflammatory activity. Combination of the UV and MS data allowed the characterization of 17 compounds, which could be classified into flavonoid glycosides or hydroxycynnamic acid derivatives. Six of them were isolated using preparative HPLC: isoscutellarein 7-O-[6′″-O-acetyl- β -D-allopyranosyl-(1→2)]- β -D-glucopyranoside, 4′-O-methylhypolaetin 7-O-[6′″-O-acetyl- β -D-allopyranosyl-(1→2)]- β -D-glucopyranoside, hypolaetin 7-O-[6′″-O-acetyl- β -D-allopyranosyl-(1→2)]-6″-O-acetyl- β -D-glucopyranoside, 4′-O-methylisoscutellarein 7-O-[6′″-O-acetyl- β -D-allopyranosyl-(1→2)]- β -D-glucopyranoside, isoscutellarein 7-O-[6′″-O-acetyl- β -D-allopyranosyl-(1→2)]-6″-O-acetyl- β -D-glucopyranoside and 4′-O-methylhypolaetin 7-O-[6′″-O-acetyl- β -D-allopyranosyl-(1→2)]-6″-O-acetyl- β -D-glucopyranoside. The presence of phenylpropanoid glycoside martynoside was reported for the first time in the wild growing S. raeseri subsp. raeseri. Hypolaetin derivatives, known for their antiinflammatory activity, dominated and were more abundant in S. raeseri subsp. raeseri grown in NPG in comparison with S. scardica grown nearby the NPG. The type of solvent affected distribution and the amount of bioactive compounds and the advantage was given to less polar extracts which were richer in hypolaetin derivatives.

Keywords: Sideritis ; polar extracts; polyphenols; LC/MS.