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Anticancer and Alkaline Phosphatase Inhibitory Effects of Compounds Isolated from the Leaves of Olea ferruginea Royle

Muhammad Ali Hashmi, Hamid Saeed Shah, Afsar Khan, Umar Farooq, Jamshed Iqbal, Viqar Uddin Ahmad and Shagufta Perveen

Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan;

Center for Advance Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan;

H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

Department of Pharmacognogy, College of Pharmacy, King Saud University, Riyadh, P. O. Box 2457, Riyadh 11451, Saudi Arabia

Abstract: One flavonoid, one ursane type triterpene, and two seco-iridoids were isolated from the leaves of Olea ferruginea Royle. The compounds were screened against TNALP and CIALP enzymes for their in vitro alkaline phosphatase inhibitory studies and HeLa cancer cell lines to measure their anticancer potential. Compound 1 showed the highest activity of 89.5 ± 1.5 nM against CIALP enzyme. All the compounds showed little activity against TNALP enzyme which shows the specificity of these compounds for CIALP enzyme only. Compounds 1, 3 , and 4 exhibited anticancer activity comparable to the reference drug vincristine (VNCT). All the compounds showed minimum toxicity against vero cells at 10 μM concentration.

Keywords: Olea; Olea ferruginea; secoiridoid; anticancer; flavonoid . © 2015 ACG Publications. All rights reserved.