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Antioxidant Activity of Chemical Constituents Isolated from Pithecellobium clypearia

Lingzhi Li, Ying Peng, Ch ong Hu, Ch unting Liu, Qingbo Liu, Linguang Li, Jie Wu and Shaojiang Song

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China

Key Laboratory of Structure-Based, Drug Design ＆ Discovery, (Ministry of Education), Shenyang 110016, Liaoning, P. R. China

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, PR China

Abstract: Phytochemical investigation of the aqueous extract of Pithecellobium clypearia afforded one n ovel compound, clypearoside A(1), and one new stereoisomer , (-)-(2S,3S)-epigallocatechin-7-gallate(2) and four known ones (3-6). Their structures were elucidated on the basis of spectroscopic data, especially 2D NMR, HRESIMS and ECD spectra. Compounds 2-4 showed strong activity against DPPH (diphenylpicrylhydrazyl) and ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonate) with an IC 50 value of 11.9-13.2 μg/mL (DPPH) and 4.5-6.4 μg/mL (ABTS).

Keywords: Pithecellobium clypearia; DPPH and ABTS radical scavenging assay; ECD spectra. © 2015 ACG Publications. All rights reserved.