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Stemona alkaloids isolated from Stemona tuberosa roots and their inhibitory activity on lipopolysaccharide-induced nitric oxide production

Ki Yong Lee, Eun Ju Jeong, Sang Hyun Sung and Young Choong Kim

College of Pharmacy, Korea University, Sejong 339-700, South Korea

College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, South Korea

Department of Agronomy & Medicinal Plant Resources, College of Life Sciences and Natural Resources, Gyeongnam National University of Science and Technology, Jinju, South Korea

Abstract: A methanol extract of the roots of Stemona tuberosa (Stemonaceae) significantly inhibited lipopolysaccharide-induced nitric oxide production in murine BV2 microglial cells. Eight alkaloids tuberostemonine K (1), neotuberostemonine (2), oxotuberostemonine (3), bisdehydroneotuberostemonine (4), epibisdehydrotuberostemonine J (5), bisdehydrotuberostemonine (6), tuberostemoninol (7), and neostenine (8) were isolated from the methanolic extract using bioactivity-guided fractionation. Among them, compounds 4, 5 and 6 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia at 100 m M.

Keywords: Stemona tuberose ; alkaloids ; lipopolysaccharide ; BV2 microglia ; nitric oxide . © 2015 ACG Publications. All rights reserved.