7.

Secondary Metabolites and Bioactivity of the Endophytic Fungus Phomopsis theicola from Taiwanese endemic plant

Yi Hsiao, Hsun-Shuo Chang, Ta-Wei Liu, Sung-Yuan Hsieh, Gwo-Fang Yuan, Ming-Jen Cheng, and Ih-Sheng Chen

Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan

School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan

Abstract: A new cytochalasan named as phomocytochalasin (1), together with five previously identified compounds, cytochalasin H, cytochalasin N, RKS-1778, dankasterone B, cyclo(L-Ile-L-Leu), were isolated from the solid fermentate of Phomopsis theicola BCRC 09F0213, an endophytic fungus isolated from the leaves of an endemic Formosan plant Litsea hypophaea Hayata . The structure of the new compound was established by spectroscopic methods, including UV, IR, HR-ESIMS, and extensive 1D- and 2D-NMR techniques. Among the isolates, cytochalasin N showed NO inhibitory activity with IC 50 values of 77.8 μM . Cytochalasin H showed the progesterone receptor (PR) antagonism with the IC 50 value of 1.42 μM.

Keywords: Phomopsis theicola ; endophytic fungus; Litsea hypophaea; phomocytochalasin. © 2015 ACG Publications. All rights reserved.