Anatolian Propolis Prevents Oxalate Kidney Stones: Dramatic Reduction of Crystal Deposition in Ethylene-Glycol-Induced Rat Model
Fehmi Narter, Ayça Diren, Alper Kafkaslı, Allison P. Eronat, M. Fatih Seyhan, Hülya Yılmaz-Aydoğan, Sukran Sarıkaya,
Seda Damla Hatipoğlu, Kemal Sarıca and Oguz Ozturk
Department of Urology, Kartal Dr.Lütfi Kırdar Research and Training Hospital, Istanbul, Türkiye
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Türkiye
Department of Urology, Istanbul Yeni Yuzyil University, Istanbul, Türkiye
Department of Pathology, Kartal Dr.Lütfi Kırdar Research and Training Hospital, Istanbul, Türkiye
Reference Materials Laboratory, Chemistry Group, National Metrology Institute, TUBITAK UME, Gebze-Kocaeli, Türkiye
Abstract: One of the many properties of propolis, a gift of nature, is that it is a potent antioxidant agent, which has been shown to be a miracle-worker in many different diseases. In this study, its possible protective and reversing effects against hiperoxaluria was investigated in a rat model in comparison with verapamil. In all 5 groups (Total n=76), aside from the control, hiperoxaluria was induced with continuous ethylene glycol (EG) administration. The others received EG only, 50 mg/kg propolis, 100 mg/kg propolis and 1mg/kg verapamil. To estimate the antioxidant/oxidant status in the tissue and serum samples, catalase (CAT), superoxide dismutase (SOD), total glutathione (GSH), nitric oxide (NO), malonyl dialdehyde (MDA) and total anti-oxidant capacity (T-AOC) were measured after 7 and 28 days. In the early phase, serum T-AOC levels were significantly elevated in the EG+P100 (p=0.0062) compared to the control, while in the late phase, it was elevated in the EG+P50 (p=0.037) and EG+V (p=0.009) compared to the EG only group. Propolis administration was observed to dramatically decrease crystal deposition (p<0.0001) and was more effective in the prevention of oxalate-induced renal injury than verapamil. Propolis being a natural product with almost none adverse effects elevates its value as a future approach to urolithiasis.
Keywords: Hyperoxaluria; propolis; verapamil; antioxidant; crystal deposition. © 2018 ACG Publications. All rights reserved.