Identification and Quantification, by NMR and LC-MS, of Sterols Isolated from the Marine Sponge Aplysina aerophoba
Pere Ferriol, Francisco J. Toledo Marante, Ignacio Brouard Martin, José J. Santana Rodríguez, Rayco Guedes Alonso, Alba González Benkovics and Roberto Mioso
Department of Chemistry, University of Las Palmas de Gran Canaria, Gran Canaria 35017, Spain
Institute of Natural Products and Agrobiology (IPNA), CSIC, Tenerife, 38206, Spain
University Institute for Environmental Studies and Natural Resources (i-UNAT), University of Las Palmas de Gran Canaria, 35017, Spain
Department of Biochemistry, Federal University of Pernambuco, Recife, PE, 50670-901, Brazil
Abstract: Aplysterol, several didehydroaplysterols, and their acetylated derivatives were isolated from samples of the marine sponge Aplysina aerophoba, which had been collected on the coast of the Canary Islands, and were identified by chromatography and 1H- and 13C-NMR spectroscopy. A quantitative analysis was done, based on the relative intensities of the signals corresponding to the olefinic quaternary carbons from the 13C-NMR spectrum at 125 MHz. Finally, the results described were confirmed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
Keywords: Aplysina aerophoba ; marine sponge; sterols; quantitative analysis; NMR; UHLC-MS/MS. © 2018 ACG Publications. All rights reserved.