Records of Natural Products

Year: 2018 Volume: xx   Issue: x

 

  ORIGINAL ARTICLE

1.

Identification and Quantification, by NMR and LC-MS, of Sterols Isolated from the Marine Sponge Aplysina aerophoba

Pere Ferriol, Francisco J. Toledo Marante, Ignacio Brouard Martin, José J. Santana Rodríguez, Rayco Guedes Alonso, Alba González Benkovics and Roberto Mioso

 Department of Chemistry, University of Las Palmas de Gran Canaria, Gran Canaria 35017, Spain

Institute of Natural Products and Agrobiology (IPNA), CSIC, Tenerife, 38206, Spain

University Institute for Environmental Studies and Natural Resources (i-UNAT), University of Las Palmas de Gran Canaria, 35017, Spain

Department of Biochemistry, Federal University of Pernambuco, Recife, PE, 50670-901, Brazil

 Abstract: Aplysterol, several didehydroaplysterols, and their acetylated derivatives were isolated from samples of the marine sponge Aplysina aerophoba, which had been collected on the coast of the Canary Islands, and were identified by chromatography and 1H- and 13C-NMR spectroscopy. A quantitative analysis was done, based on the relative intensities of the signals corresponding to the olefinic quaternary carbons from the 13C-NMR spectrum at 125 MHz. Finally, the results described were confirmed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).

Keywords: Aplysina aerophoba ; marine sponge; sterols; quantitative analysis; NMR; UHLC-MS/MS. © 2018 ACG Publications. All rights reserved.