Biological Activity and Chemical Composition of the Endophytic fungus Fusarium sp. TP-G1 obtained from the Root of Dendrobium officinale Kimura et Migo
Sanjun Shi, Yuanyuan Li, Yue Ming, Chenwen Li, Ziwei Li, Jianhong Chen and Minghe Luo
Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Abstract: Dendrobium officinable Kimura et Migo is a famous traditional herb medicine and is wildly used in folk as a tonic herb. Its endophytic fungi play prominent part in the function of D. officinable Kimura et Migo. In this study we first report the co-production of trichosetin (1), beauvericin (2), beauvericin A (3), enniatin B (4), enniatin H (5), enniatin I (6), enniatin MK1688 (7), fusaric acid (8) and dehydrofusaric acid (9) by the endophytic fungus Fusarium sp. TP-G1 deriving from the root of D. officinable kimura et Migo. These isolates’ reported bioactivities and the tested biological activities in this paper consisted with the functions of D. officinable Kimura et Migo. We found the MICs value of compounds 1−7 against Staphylococcus aureus and MRSA showed nearly no difference, while compounds 8−9 were first found to have the antimicrobial activities against Acinetobacter baumannii with a MIC value of 64 μg/mL and 128 μg/mL respectively. Compounds 2, 4, 5 and 6 were first found to show moderate anti-tumor activities against BL16F10; and compounds 4 and 5 were first found to show moderate anti-tumor activities against MCF-7. Furthermore, we report first here the isoleucine (Ile) replacement in beauvericin and enniatin will influence their antibacterial and brine shrimp lethality activities.