4.

Chemical Constituents and Antioxidant Activity of Geranium wallichianum

Mohammad Ismail, Mohammad Ibrar, Zafar Iqbal, Javid Hussain, Hidayat Hussain, Manzoor Ahmed, Asma Ejaz and Muhammad I. Choudhary

Department of Pharmacy, University of Peshawar, NWFP, Pakistan

Department of Chemistry, Kohat University of Science and Technology N.W.F.P, Pakistan

Department of Chemistry, Universität Paderborn, Warburger Straße 100, 33098 Paderborn, Germany

Department of Chemistry, University Malakand, N.W.F.P, Pakistan

H. E. J Research Institute of Chemistry, International Center for Chemical and Biological  Sciences, University of Karachi, Karachi-75270, Pakistan.

Abstract: The study of the chemical constituents of the whole plant of Geranium wallichianum (Geraniaceae) has resulted in the isolation and characterization of six compounds. These six compounds were identified as ursolic acid (1), β-sitosterol (2), stigmasterol (3),b-sitosterol galactoside (4), herniarin (5), and 2,4,6-trihydroxyethylbenzoate (6) which were isolated for the first time from Geranium wallichianum. The above compounds were individually identified by spectroscopic analyses and comparisons with reported data. The antioxidant potential of Geranium wallichianum extracts has been investigated by DPPH radical scavenging assay and EtOAc extract was found to be most potent with IC50 19.05 ug/mL.

Keywords: Geranium wallichianum; antioxidant activity; DPPH, coumarin; triterpenoid; steroid.