3. |
Antioxidant Properties and Total Phenolic Content of Three Varieties of Carob Tree Leaves from Morocco
Hanane El Hajaji, Nadya Lachkar, Katim Alaoui, Yahya Cherrah, Abdellah Farah, Abdesslam Ennabili, Brahim El Bali and Mohammed Lachkar
Laboratoire d’Ingénierie des Matériaux Organométalliques et Moléculaires ‘’LIMOM’’, Unité associée au CNRST (URAC 19), Faculté des Sciences, Université Sidi Mohamed Ben Abdellah, B.P.1796 (Atlas), 30000 - Fès, Morocco
Laboratoire de Pharmacologie et Toxicologie, Faculté de Médecine et de Pharmacie, Université Mohamed V, Rabat-Souissi, Rabat, Morocco
National Institute of Medicinal and Aromatic Plants, University of Sidi Mohamed Ben Abdellah ,
BP 8862, 30100 - Fès, Morocco
Laboratory of Mineral Solid and Analytical Chemistry, ‘’LMSAC’’, Department of Chemistry, Faculty of Sciences, University Mohamed I, Po. Box 717, 60000 Oujda, Morocco
Abstract: The in vitro antioxidant activity and the total phenolic content (Folin−Ciocalteu method) of three successive extracts of three varieties of Ceratonia siliqua L. leaves ( grafted female, spontaneous female, spontaneous male) grown in Morocco were investigated by using in-vitro antioxidant models including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity . T he global polyphenols concentration ranged from 0.45 to 2.64 (g/L GAE) in the three categories of the extracts . In each variety, ethyl acetate fraction exhibited the highest antioxidant activity compared to other fractions. Grafted female trees globally showed a higher polyphenols concentration than the spontaneous female and spontaneous male ones. Our results clearly demonstrate that all extracts have antioxidant capacity. Among the categores, the ethyl acetate extracts of carob tree leaves exhibited strong scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) than the diethyl ether and dichloromethane extracts. Carob leaf extracts contain high amounts of polyphenols with strong antiradical, antioxidant capacity and reducing properties which might constitute an important source of natural antioxidants.
Keywords: Antioxidant activity; Phenolic content; Ceratonia siliqua L. |