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Lignan Glycosides from Gentianella Acuta (Michx.) Hulten. and Their Protective Effects Against H 2O 2-Induced Apoptosis in H9c2 Cardiomyoblasts

Ying Yu, Zhi-Bin Wang, Ya-Dong Zhai, Pu-Yuan Song, Qiu-Hong Wang, Bing-You Yang and Hai-Xue Kuang

Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China

Abstract: T wo new lignan glycosides named acutosides A (1) and B (2) have been isolated from the whole plant of Gentianella acute (Michx) Hulten. and t heir structures were determined as (-)-(7 R ,8S,7'E)-4-hydroxy-3, 3 '-dimethoxy-7,4'-epoxy-8, 5 '-neolign-7'-ene-9,9'-diol 9'-ethyl ether -4-O-β-D- glucopyranosid e and (-)-(7 R ,8S,7'E)-4-hydroxy-3, 3 '-dimethoxy-7,4'-epoxy-8, 5 '-neolign-7'-ene-9,9'-diol 9-ethyl ether -9 ' -O-β-D- glucopyranosid e by means of 1D-, 2D-NMR , HR -ESI -MS , CD techniques and chemical reactions. Along with the above two new compounds we got six known ones: (7R,8S)-dehydrodiconiferyl alcohol-4,9 ' -di-O-β-D- glucopyranosid e (3 ), alaschanisoside A (4 ), citrusin A (5 ) , 8,4',9'-trihydroxy-3,3'-dimethoxy-9,7'-epoxylignan 4-O- β -D- glucopyranosid e (6 ), l eptolepisol D (7 ) and acanthoside D (8 ). The cardioprotective effects of compounds 1-8 were evaluated by measuring the viability of the compounds 1-8 pretreated H9c2 cardiomyoblasts after exposure to H 2O 2-induced apoptosis, the results showed that compounds 1,3,6,8 which could increase cell viability >15% at 200 μM had remarkably protective influence against H 2O 2-induced apoptosis. All the compounds were tested for cytotoxicity against two human tumor cell lines: gastric cancer SGC-7901 and breast cancer MCF-7. But they were inactive (IC 50>40 μM).

Keywords: Lignan glycosides; Gentianella acute (Michx.) Hulten. ; H9c2 Cardiomyoblasts; cardioprotective activity. © 2014 ACG Publications. All rights reserved.