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Evaluation of Anti-inflammatory and Immunosuppressive Properties of Cynanchum ascyrifolium Matsum. and its Active Secondary Metabolites Chao-Feng Zhang, , Xiu-Fen Liu,Yan-Yan Zhao and Mian Zhang Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210098 Department of Pharmacy, Yantai University, Yantai 264005 P. R. China Abstract: The ethanol extract of Cynanchum ascyrifolium Matsum. showed significant anti-inflammatory and immunosuppressive activities with dose dependent manner using in vitro experiments. Then, the bioassay-guided ethanol extract lead to identification of four active compounds, namely ursolic acid (1), maslinic acid (2), cynanoside A (3) and syringaresinol (4). Among these compounds, cynanoside A (3), a C21 steroid, exhibited the highest anti-inflammatory and immunosuppressive effect on the Con-A or LPS activated lymphocytes at the concentration of 1 m M (P<0.01 vs model group). The results provided experimental evidence for the traditional use of C. ascyrifolium Matsum. in treating various disease associated with inflammation. Keywords: Cynanchum ascyrifolium Matsum.; anti-inflammation; immunosuppression; cynanoside A. © 2015 ACG Publications. All rights reserved. |