A new HPLC-UV method for the simultaneous measurements of α-escin and β-escin in creams containing Aesculus hippocastanum L. extract

Original Article

JOURNAL 2985

Journal of Chemical Metrology

VOLUME & ISSUE

Year: 2023 Issue: 2 July-December

PAGES

p.249 - 256

DOI ADDRESS

http://doi.org/10.25135/jcm.101.2310.2985

STATISTICS

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AUTHORS

Burhan Ceylan

GRAPHICAL ABSTRACT

ABSTRACT

Amount of α-escin and β-escin in cosmetic cream formulations were determined by simple reverse phase HPLC method by using the C18 column at 228 nm. The mobile phase is composed of acetonitrile-water (60:40) at pH 2.4. The method validation study was carried out based on the ICH recommendations and linear regression equations were determined as y=1548.3x+340.7 and y=1521.3x-91.9, for the analytes, respectively. While LOD values of the method were determined for α-escin and β-escin as 0.003 µg/mL and 0.027 µg/mL, LOQ values were determined as 0.01 µg/mL and 0.08 0.01 µg/mL, respectively

KEYWORDS

Horse chesnut
α-escin
β-escin
HPLC-UV
Validation