JOURNAL 3547
Records of Agricultural and Food Chemistry
Year: 2025 Issue: 1 January-June
p.86 - 93
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GRAPHICAL ABSTRACT
ABSTRACT
The phenolic content of the decoction and infusion extracts of Origanum onites from three different locations in Türkiye was evaluated using LC-MS/MS. The antioxidant capacity of the decoction and infusion was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH), β-carotene linoleic acid, and CUPRAC assays. Additionally, the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of the both extracts of O. onites were investigated. The highest level of rosmarinic acid (935.79 mg/kg), penduletin (743.53 mg/kg), and kaempferol (506.14 mg/kg) were found in the decoction extract of O. onites from Kemer. In addition, Kemer had the highest DPPH free radical scavenging (61.43-75.59%) and lipid peroxidation (59.03-78.99%) activity of all the locations examined. Kazdağı sample had the highest value on the CUPRAC assay (3.83 mmol TR g-1 for decoction and 3.20 mmol TR g-1 for infusion, respectively). The study concludes that a quantitative relationship between phenolic content and antioxidant activity was established, which is important for understanding the role of phenolics in antioxidant activity. The location had an impact on the biological activities of both extracts of O. onites, as well as their phenolic content.
KEYWORDS- Origanum onites
- different location
- phenolics
- antioxidant
- AChE
- BChE
