JOURNAL 743


Records of Natural Products
VOLUME & ISSUE
Year: 2014 Issue: 3 July-September
PAGES
p.286 - 289
STATISTICS
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AUTHORS
    Paul Coulerie, Alexandre Maciuk, Cécilia Eydoux, Edouard Hnawia, Nicolas Lebouvier, Bruno Figadère, Jean-Claude Guillemot and Mohammed Nour
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GRAPHICAL ABSTRACT


ABSTRACT


Since a few decades the dengue virus became a major public health concern and no treatment is available yet. In order to propose potential antidengue compounds for chemotherapy we focused on DENV RNA polymerase (DENV-NS5 RdRp) which is specific and essential for the virus replication. Carpolepis laurifolia belongs to the Myrtaceae and is used as febrifuge in traditional kanak medicine. Leaf extract of this plant has been identified as a hit against the DENV-NS5 RdRp. Here we present a bioguided fractionation of the leaf extract of C. laurifolia which is also the first phytochemical evaluation of this plantFive flavonoids, namely quercetin (1), 6-methyl-7-methoxyapigenin (2), avicularin (3), quercitrin (4) and hyperoside (5), together with betulinic acid (6), were isolated from the leaf extract of C. laurifolia. All isolated compounds were tested individually against the DENV-NS5 RdRp and compared with four other commercial flavonoids: isoquercitrin (7), spiraeoside (8), quercetin-3,4’-di-O-glucoside (9) and rutine (10). Compounds 3, 4, 6, 8 and 10 displayed IC 50 ranging from 1.7 to 2.1 µM, and were the most active against the DENV-NS5 RdRp.

KEYWORDS
  • Carpolepis laurifolia
  • Dengue Virus
  • Flavonoids
  • Triterpenes.