JOURNAL 493


Records of Natural Products
VOLUME & ISSUE
Year: 2015 Issue: 1 January-March
PAGES
p.146 - 152
STATISTICS
Viewed 1998 times.
AUTHORS
    Gihan O. M. ELhassan, Achyut Adhikari, Omer M. Abdalla, Ahmed Shukrulla, Asaad Khalid, M. Iqbal Choudhary, Mohammed A. Mesaik and Sakina Yagi
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


Phytochemical investigation on the stem of Euphorbia polyacantha Boiss. (Euphorbiaceae) afforded a new anthraquinone named as 1, 8-dihydroxy -3- (hydroxyl methyl) -5- methoxy anthracene- 9,10- dione (1) along with three known compounds, aloe emodin (2) , 3, 4, 3 ¢ –tri-O-methyl ellagic acid (3), and 3,3 ¢ ,4, 4 ¢ - tetra-O-methyl ellagic acid (4). Their structures were determined on the basis of IR, 1D- and 2D- NMR spectroscopy and mass spectrometry analysis. The effect of isolated compounds on oxidative burst of neutrophils and T-cell proliferation response was studied. Compounds 3 and 1 displayed strong oxidative burst inhibitory activity with IC 50 2 ± 0 and 3.5 ± 1.1 µg/mL respectively, whereas they moderately inhibited the T-cell proliferation with IC 50 28.5 ± 1.8 and 22.5 ± 2.0 µg/mL respectively. Compound 2 was relatively inactive with IC 50 43.2 μg/mL in oxidative burst and >50 μg/mL in case of T-cell proliferation. This is the first report on the phytochemical profile and biological activity of E. polyacantha.

KEYWORDS
  • Euphorbia polyacantha
  • anthraquinone
  • immunomodulation
  • oxidative burst
  • T-cell proliferation