Anti-urease Secondary Metabolites from Seriphidium quettense

Ethyl acetate layer of the methanolic extract of Seriphidium quettense was subjected to silica gel column chromatography to isolate one new; seriphiloid (1), and four known compounds; ilicic acid (2), 6 a -hydroxy-8(10)-oplopen-14-one (3), 2-(4-hydroxyphenyl)-5,6,7-trimethoxy-4H-chromen-4-one (4) and 2-(3,4-dihydroxyphenyl)-5,6,7-trimethoxy-4H-chromen-4-one (5). The chemical structure of the new isolate was established with the help of 1D, 2D NMR techniques and high resolution mass spectrometry. Known compounds were identified because of 1D NMR and mass spectrometric analysis and in comparison with the literature values. Compounds 1-5 were evaluated for their acetylcholinesterase, butyrylcholinesterase, a -glucosidase and urease inhibitory activities. Most of the metabolites were found inactive; however, compounds 2 and 3 showed good antiurease activity with IC 50 value 21.5±0.1 and 20.8±0.1 µg/mL, respectively.