JOURNAL 59


Records of Natural Products
VOLUME & ISSUE
Year: 2018 Issue: 4 July-August
PAGES
p.332 - 339
STATISTICS
Viewed 1282 times.
AUTHORS
    Yuehui Lan, Xiaofeng Chi , Guoying Zhou and Xiaohui Zhao
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


An efficient method for the screening and isolation of potent antioxidants from a tibetan medicinal plant by employing online HPLC–DPPH assay combined HSCCC has been successfully established. Six major constituents : boschnaloside ( 1 ), alyssonoside ( 2 ), leucosceptoside A ( 3 ), isoverbascoside ( 4 ), leucosceptoside B ( 5 ) and verbascoside ( 6 ) were isolated and purified from the water extract by HSCCC using ethyl acetate-n-butanol-water as two-phase solvent system. The results indicated that the combination of the online HPLC–DPPH assay with HSCCC could be suitable for the screening and separation of antioxidant compounds from extract of this plant or other medicine plants.

KEYWORDS
  • Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong
  • online HPLC–DPPH
  • HSCCC
  • antioxidants