Phytochemical and Biological Evaluation of Ultrasound-Assisted Spray Dried Lonicera etrusca for Potential Management of Diabetes

Honeysuckle or Lonicera etrusca is one of the important medicinal-plants that is traditionally-used as a potential antioxidant. The aim of this work was to make a phytochemical-evaluation of Lonicera etrusca ultrasound-assisted spray-dried extract versus conventional-method utilizing RP-HPLC aided-method in the potential management of diabetes. Ultrasound-assisted spray-drying (US-SD) is a new productive-technique aiming at increasing the efficiency and extract-yield in addition to short time of exposure to elevated temperature thus improving product stability. A bio-guided study utilizing RP-HPLC, 1H and 13C NMR methods indicated that the most active antidiabetic-compound was isochlorogenic acid (ICA). Diabetes measurement utilizing glucometers and HbA1c methods was applied. Serum-insulin levels and serum-catalase (CAT) was also monitored. The US-SD preserved time by 4 folds and with higher yield (ca. 20%) than the conventional method. The US-SD also had higher quality in US-SD honeysuckle (HS-sd) constituents than the conventional method as supported by RP-HPLC analysis. Rotary evaporated honeysuckle (HS-r) major peaks identified were; chlorogenic acid (38.6%), caffeic acid (5.8%), isochlorogenic acid (36.1%), luteolin-7-O-glucoside (3.3%), and quercetin (3.0%). HS-sd major peaks identified were; chlorogenic acid (40.5%), caffeic acid (6.4%), isochlorogenic acid (43.3%), luteolin-7-O-glucoside (3.5%), and quercetin (3.2%). HS-sd had significant (p˂0.05, n=7/group) antidiabetic-activity more than HS-r. HS-sd had more-significant dose-dependent increase in serum-insulin, CAT-levels and body-weights more prominent than HS-r. Compared to conventional-methods, US-SD has shown to be time-conserving, efficient, and active-ingredients preserving method. The antidiabetic-potentials of HS-sd and ICA were probably mediated via the significant insulin-secretagogue effect and the attenuation glucose-provoked oxidative-stress.