JOURNAL 86


Records of Natural Products
VOLUME & ISSUE
Year: 2018 Issue: 6 November-December
PAGES
p.595 - 601
STATISTICS
Viewed 1382 times.
AUTHORS
    Murat Türkoğlu, Songül Kılıç, Erkin Pekmezci and Murat Kartal
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GRAPHICAL ABSTRACT


ABSTRACT


Lepidium sativum L. (Brassicaceae) (Ls), garden cress, is an edible herb that is botanically related to watercress and mustard, sharing their peppery, tangy flavor and aroma. The various parts of the plant have been traditionally used for many respiratory, rheumatologic, cardiovasculary, metabolic, and gastrointestinal disorders. Several studies were performed showing its antiinflammatory, anticarcinogenic, antiproliferative as well as tissue protective effects. In all of these studies the positive effects of Ls were concluded as the result of its antioxidant features due to the polyphenols and organosulphur compounds. In this study, we investigated the molecular basis of antiinflammatory, antiproliferative, antimitotic and antiandrogenic effects of Ls leaf extract in HaCaT cells. The gene expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α), interleukin 1-alpha (IL-1α), and 5 alpha-reductase type II (SRD5A2) were studied. Gene expressions were determined by RT-qPCR analysis. Fold change results of the gene expression analyses showed that the plant extract caused statistically significant downregulation of VEGF (0.2167, p<0.0001), TNF-α (0.4133, p = 0.0262), and SRD5A2 (0.0567, p<0.0001). The fold change for IL-1α was 0.6900 and this result was not statistically significant (p = 0.1522). These preliminary results may partially explain the clinical success of Ls in the traditional medicine. Considering these results, topical application of Lepidium sativum extract may be beneficial for inflammatory, androgen dependent disorders of the skin.

KEYWORDS
  • Lepidium sativum
  • garden cress
  • SRD5A2
  • TNFα
  • VEGF