JOURNAL 2343


Records of Natural Products
VOLUME & ISSUE
Year: 2022 Issue: 6 November-December
PAGES
p.559 - 571
STATISTICS
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AUTHORS
  • Yuan Li
  • Chunyan Zhang
  • Yiwen Nie
  • Ruifeng Zhang
  • Xiaoxiang Zhai
  • Yanjuan Duan
  • Wei Fan
  • Weiying Gu
  • Kourong Shi
  • Lan Luo
  • Jianyong Zhu
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


The ethyl acetate extraction (WCE) of Wikstroemia chamaedaphne Meisn on the viability of melanoma cell lines B16 and A375 were evaluated. The WCE exhibited a potent cytotoxic against B16 and A375 with IC50 values of 156.2 and 192.8 µg/mL, respectively. Cell migration was assessed using a wound healing assay. The WCE caused cell cycle arrest in the G0/G1 phase in B16 cells and the S phase in A375 cells, according to cell cycle analysis. Flow cytometric analysis revealed that WCE promoted apoptosis in B16 and A375 cells in a dose-dependent manner. Chemical analysis of WCE resulted in the isolation of a new diterpenoid, wikstchalin A (6) with five known flavonoids (1-5) and two known diterpenoids (7 and 8). Spectroscopic analysis revealed their structures, and CD analysis revealed the absolute configurations of 6. The absolute structure of pimelotide A (7) was firstly confirmed by single-crystal X-ray diffraction. The cytotoxicities of all the compounds (18) were evaluated against B16 cell lines. Compound 8 was more active than the positive control, dacarbazine (IC50 300 μM), in terms of cytotoxicity against B16, with an IC50 value of 7.9 μM.

KEYWORDS
  • Wikstroemia chamaedaphne
  • flavonoid
  • diterpenoid
  • antitumor activity

SUPPORTING INFORMATION


Supporting Information
Download File A4-311-RNP-2202-2343-SI.pdf (2.3 MB)