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Quantification of main secondary metabolites of Satureja icarica P.H. Davis (Lamiaceae) by LC-HRMS and evaluation of antioxidant capacities

Original Article

JOURNAL 2956

Journal of Chemical Metrology
VOLUME & ISSUE
Year: 2023 Issue: 2 July-December
PAGES
p.199 - 214
DOI ADDRESS
http://doi.org/10.25135/jcm.2311.2956
STATISTICS
Viewed 437 times.
AUTHORS
  • Betül Kiraz Kinoğlu
  • Tuncay Dirmenci
  • Saleh H. Alwasel
  • İlhami Gülçin
  • Ahmet C. Gören
PDF OF ARTICLE

GRAPHICAL ABSTRACT

ABSTRACT

In this study, we determined antioxidant activity of Satureja icarica (Lamiaceae) which is used as a spice by the local population against 1,1-diphenyl-2-picryl-hydrazyl (DPPH·), N,N-dimethyl-p-phenylenediamine radicals (DMPD•+), and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS•+) as well as their ability to reduce Fe3+, Fe3+-TPTZ, and Cu2+. The antioxidant activities of both extracts were similar to those of standards in all assays. For instance, IC50 of S. icarica were for scavenging DPPH was 10.19 μg/mL. The results of liquid chromatography-high resolution mass spectrometry (LC-HRMS) revealed that the main secondary metabolites in Satureja icarica were found to be as rosmarinic acid and luteolin-7-O-rutinoside.

 KEYWORDS
  • Satureja icarica
  • LC-HRMS
  • seconder metabolite
  • antioxidat activity
  • rosemarinic acid