UncertCalc: a web-based tool for measurement uncertainty calculation

This study presents UncertCalc, a web-based tool developed to facilitate measurement uncertainty calculations in laboratory settings. The application is supporting both Top-Down and Bottom-Up approaches. The Top-Down module performs ANOVA based estimation of within day repeatability and between day intermediate precision using multi day repeated measurements. The Bottom-Up module calculates the combined measurement uncertainty by incorporating user-defined uncertainty components. Both modules have graphical visualization of uncertainty contributions. The computational accuracy of UncertCalc was independently validated using Microsoft Excel and R, using randomly generated representative data for both Top-Down and Bottom-Up approaches. Results from these comparisons proved to be in perfect agreement across the different platforms, confirming that UncertCalc offers statistically reliable and fully traceable calculations, consistent with established analytical tools.
UncertCalc has been developed to provide a practical solution that enables a sound, accessible process of biochemical measurement methods according to ISO/IEC 17025.