JOURNAL 345


Records of Natural Products
VOLUME & ISSUE
Year: 2016 Issue: 1 January-February
PAGES
p.93 - 102
STATISTICS
Viewed 1144 times.
AUTHORS
    Gerasimia Tsasi, Marina Soković, Ana Cirić, Effimia Eriotou, Eleni Sakadani, Yiannis Samaras and Helen Skaltsa
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


The essential oils (EOs) from the leaves and the flowers of Smyrnium olusatrum L. , growing wild in the island of Cephalonia (Greece), were analyzed by GC-FID and GC-MS. Fifty nine constituents, which accounted for 90.3% (fl) and 97.1% (lvs) of the oils, were identified. Furanodiene, g ermacrone and furanoeremophil-1-one were the major constituents in both essential oils; they were also isolated from the flowers essential oil and identified using spectroscopic methods, ie. 1D and 2D NMR, GC-MS . In addition b -myrcene ( 11.7%) and b -phellandrene (5.2%) were main constituents in the essential oil of the leaves. The essential oils and the pure isolates were evaluated for antifungal activity against Aspergillus fumigatus , A. versicolor, A. ochraceus, A. niger, Trichoderma viride, Penicillium funiculosum, P. ochrochloron, P. verucosum var. cyclopium by using the microdilution method and proved to possess significant antifungal effect. Among them, (+) furanoeremophil-1-one was particularly active with MIC values in the range of 0.0008-0.125 mg/mL and MFC values of 0.025-0.050 mg/mL and proved more effective than the commercial mycotics ketoconazole and bifonazole used as positive controls.

KEYWORDS
  • Smyrnium olusatrum
  • essential oil
  • isolation
  • (+) furanoeremophil-1-one
  • furanodiene
  • (-) germacrone
  • antifungal activity .