JOURNAL 3557
Records of Natural Products
Year: 2025 Issue: 5 September-October
p.596 - 610
Viewed 73 times.
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Kuniyoshi Shimizu
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Asih Gayatri
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Fahd M. Abdelkarem
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Yhiya Amen
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Masako Matsumoto
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Maki Nagata
GRAPHICAL ABSTRACT
ABSTRACT
The development of phytochemicals from olive leaves and seeds for cosmeceutical applications is still scarce. This study aimed to perform comprehensive investigations of the bioactive compounds and biological activities of olive leaves and seeds for antioxidant, skin protection from ultraviolet B (UVB) irradiation in human dermal fibroblast (NHDF) cells from adult donors, and anti-melanogenesis in B16 melanoma cells. Two bioactive compounds were isolated from olive leaves, identified as oleuropein (1) and oleuroside (2). Additionally, one bioactive compound, pinoresinol (3) was elucidated for the first time from olive seeds. Among these compounds, pinoresinol demonstrated the most potent melanin inhibitory in B16 melanoma cells at the low concentration of 1.25 μg/mL with no cytotoxicity, decreasing the melanin content to 54 ± 5.48 %. In addition, oleuropein at 100 μg/mL inhibited melanin production to 35 ± 2.15 %, without cytotoxicity. Furthermore, oleuropein at concentrations of 100-200 μg/mL promoted cell proliferation above 120 % and exhibited skin protective activity at 200 μg/mL against UVB irradiation (250 mJ/cm2), maintaining cell viability to 76.54 ± 5.05 %. This study highlights the isolated compounds from olive leaves and seeds, which are agricultural by-products, offer potential natural sunscreen products in the future with fewer side effects.
KEYWORDS
- Natural polyphenols
- cytotoxic assay
- skin protectant
- anti melanogenesis
- cosmetic
