JOURNAL 1224


Records of Natural Products
VOLUME & ISSUE
Year: 2020 Issue: 1 January-February
PAGES
p.23 - 30
STATISTICS
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AUTHORS
  • Abdulsalam A.M. Alkhaldi
  • Arafa Musa
  • Ehab M. Mostafa
  • Elham Amin
  • Harry P. De Koning
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


Phytochemical study of the ethanolic extract of Scrophularia syriaca Benth. was attained by chromatographic and spectroscopic procedures, which resulted in isolation of eight compounds; 6-O-α-L- rhamnopyranosylcatalpol (1), scropolioside B (2), gmelinoside-L (3), 8-acetyl harpagide (4), scropolioside D (5), scropolioside D2 (6), quercetin (7) and kaempferol-3-O-rutinoside (8). The antiprotozoal activity was evaluated against Trypanosoma brucei brucei (s427-WT), Trypanosoma brucei brucei (TbAT1-B48), Leishmania major and Leishmania mexicana. Compounds 2, 5, 7 and 8 exhibited mild to moderate activities against kinetoplastid parasites compared to pentamidine positive control, the mechanism of antiprotozoal activity was predicted by the molecular docking studies on the target enzyme Trypanosoma brucei glyceraldehyde-3-phosphate dehydrogenase (TbGAPDH).

KEYWORDS
  • docking
  • iridoids
  • Leishmania
  • Trypanosoma
  • Scrophularia syriaca

SUPPORTING INFORMATION


Scrophularia Syriaca photo

a photo of Scrophularia Syriaca plant growing in northern Saudi Arabia in the flowering stage

Supporting Information
Download File A3-129-RNP-1903-1224-SI.pdf (311.31 KB)