JOURNAL 2334
Records of Natural Products
Year: 2022 Issue: 6 November-December
p.622 - 632
Viewed 1822 times.
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Xuebo Zeng
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Shengyan Qian
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Yongzhong Lu
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Yongjie Li
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Lizhuang Chen
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Yixin Qian
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Zhangjiang He
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Jichuan Kang
GRAPHICAL ABSTRACT
ABSTRACT
AA chemical investigation of secondary metabolites from the saprobic fungus Tubeufia rubra led to isolation of a novel nitrogen-containing glyceride, Rubracin A (1), and a novel eight-membered cyclic ether carboxylate methyl ester, Rubracin H (2). Together with six known compounds (3-8). The chemical structure of these new compounds were elucidated by 1D and 2D NMR and HR-ESI-MS techniques. Although, there are no observed cytotoxic effects of compound 1 against MCF-7/Dox, A549/Dox, and K562/Dox cells at oncentrations below 25 μg/mL, usage of compound 1 and doxorubicin revealed the MDR reversal via decreased IC50 values than that of sole doxorubicin application in the cell lines. A preliminary Western Blot assay indicated that the MDR reversal of compound 1 was due to suppression of P-glycoprotein (P-gp) expression.
KEYWORDS- Rubracin A
- Tubeufia rubra PF02-2
- cytotoxic activity
- MDR reversal
