Records of Natural Products

Year: 2016 Volume: 10 Issue:6 November-December

Original Article

1) Determination of Volatile Organic Compounds in Fourty Five Salvia Species by Thermal Desorption-GC-MS Technique

Rec. Nat. Prod. (2016) 10:6 ; 659 - 700
by Seda Damla Hatipoglu, Nihal Zorlu, Tuncay Dirmenci, Ahmet C. Goren, Turan Ozturk and Gulacti Topcu

Volatile organic compounds (VOC) from dried aerial parts (flowers, leafs, leafy branches and stems) of fourty five Salvia species, harvested from different regions of Turkey, were determined using thermal desorption technique coupled to gas chromatography-mass spectrometer (TD-GC-MS). Total percentages of the detected volatile organic compounds of the Salvia species ranged from 70.30 to 99.65% . Total yield of VOC was found to be highly variable among Salvia species, and the percentage of each component also exhibited high variation. While sesquiterpene hydrocarbons represented 0.90-45.02% of the volatile organic compounds, the others were found to be monoterpene hydrocarbons 0.00-32.25%, monoterpenoids 0.94- 53.60%, sesquiterpenoids 0.00- 31.45% and hydrocarbons and derivatives 2.99-46.87%. The rest of the composition consisted of diterpene alcohols, fatty acids, phenolics and triterpenes. In total, 108 volatile compounds were identified from studied 45 Anatolian Salvia species.

Keywords
Volatile organic compounds Salvia chemical composition thermal desorption GC-MS cluster analysis.
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Original Article

2) Antioxidant and α-Glucosidase Inhibitory Activities of Isolated Compounds from Ipomoea aquatica

Rec. Nat. Prod. (2016) 10:6 ; 701 - 707
by Umar Lawal, Khozirah Shaari, Intan Safinar Ismail, Alfi Khatib and Faridah Abas

Ipomoea aquatica Forsk is a green leafy vegetable that is a rich source of amino acids and vitamins. Antioxidant and α-glucosidase inhibitory activities of the hexane (IAHE) and methanol (IAME) fractions of the vegetable portion of I. aquatica were investigated. The IAME fraction exhibited a strong scavenging effect of the 2,2-diphenyl-2-picryl hydrazyl (DPPH) free radicals, and this fraction contained the highest phenolic contents. Phytochemical investigation of the IAME fraction yielded three known compounds, namely 3,5-di-O-caffeoylquinic acid (1), 4,5-di-O-caffeoylquinic acid (2) and quercetin 3-O-β-D-glucoside (3). The structures were unambiguously elucidated based on 1D and 2D-NMR analyses (1H, 13C, COSY, HSQC, HMBC) and mass spectrometry data. Compound 3 was isolated for the first time from this plant.

Keywords
Antioxidant activity total phenolic content α-glucosidase inhibition spectral analysis
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Original Article

3) A New Sesquiterpenoid Derivative from the Coastal Saline Soil Fungus Aspergillus fumigatus

Rec. Nat. Prod. (2016) 10:6 ; 708 - 713
by Desheng Liu, Yuling Huang, Chunmin Li, Liying Ma, Xiaohong Pan, Daneel Ferreira and Weizhong Liu

A new sesquiterpenoid derivative, named aspergiketone (1), along with seven known compounds (2-8) were isolated from the coastal saline soil fungus Aspergillus fumigatus. Their structures were elucidated by spectroscopic analysis, and by comparison of experimental and reported data. The absolute configuration of compound 1 was defined by X-ray diffraction analysis. Compound 1 was cytotoxic towards HL-60 and A549 cell lines with IC 50 values of 12.4 and 22.1 μ M , respectively.

Keywords
Secondary metabolites Aspergillus fumigatus cytotoxic activity coastal saline soil
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Original Article

4) Guaiane-type Sesquiterpene Lactones from Chrysophthalmum montanum

Rec. Nat. Prod. (2016) 10:6 ; 714 - 720
by Perihan Gürbüz, Şengül Dilem Doğan, Leyla Paşayeva and Mehmet Yavuz Paksoy

Four new guaiane-type sesquiterpene glycosides (1-4), 4α-hydroxy-guai-1(10)-en-12,8α-olide 2β-O-β-D-glucopyranoside (1), 4α-hydroxy-guai-1(10)-en-12,8α-olide 2β-O-(6΄-trans-caffeoyl)-β-D-glucopyranoside (2), 10α-hydroxy-guai-1(2)-en-12,8α-olide 4α-O-β-D-glucopyranoside (3) and 10α-hydroxy-guai-12,8α-olide 4β-O-β-D-glucopyranoside (4) and three known guaiane-type sesquiterpenes (5-7), 4α,6α-dihydroxy-1β,5α,7αH-guaia-9(10),11(13)-dien-12,8α-olide (5), 6α-acetoxy-4α-hydroxy-1βH-guaia-9(10),11(13)-dien-12,8α-olide (6), and 4β-10α-Dihydroxy-5α-guai-1(2),11(13)-dien-12,8α-olide (7) were isolated from the aerial parts of Chrysophthalmum montanum (DC) Boiss. The structures of the compounds were elucidated by extensive 1D- and 2D-NMR spectroscopic analysis in combination with MS experiments.

Keywords
Chrysophthalmum montanum Asteraceae sesquiterpene lactone glycosides guaianolides.
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Original Article

5) Analgesic Potential of Opuntia dillenii and Its Compounds Opuntiol and Opuntioside Against Pain Models in Mice

Rec. Nat. Prod. (2016) 10:6 ; 721 - 734
by Faheema Siddiqui, Lubna Abidi, Lubna, Ching Fidelis Poh, Sabira Naqvi, Shaheen Faizi and Ahsana Dar Farooq

Opuntia dillenii (Nagphana) traditionally used against inflammation and also possess analgesic effect. Thus in the present study analgesic properties of O. dillenii cladode methanol extract, its fractions obtained via vacuum liquid chromatography along with isolated α-pyrones, opuntiol and its glucoside, opuntioside were analyzed. The acetic acid-induced writhes were reduced by O. dillenii test agents with opuntioside being most effective (IC 50 26 ± 0.9 mg/kg) and equipotent to diclofenac and β-sitosterol. Consistently, it also elicited most potent effect (IC 50: 28 ± 1.1 and 24 ± 1.2 mg/kg) during early and late phases of formalin-induced paw licking, producing effect similar to diclofenac and indomethacin. It was also most effective in hot plate test. Naloxone (opioid antagonist) reversed the analgesic effects of extract and fractions but failed to antagonize the opuntiol and opuntioside analgesic effects. In conclusion, edible O. dillenii extract, its fractions, opuntiol and opuntioside reduced peripheral and centrally mediated pain via opioid dependent and independent systems. Among them opuntioside emerged as most effective analgesic possibly due to the presence of glucose moiety at position 7 of its α-pyrone ring. This is the first report of opuntiol and opuntioside analgesic effect which may serve as lead compounds in designing of new analgesics.

Keywords
Opuntia dillenii opuntiol opuntioside analgesic inflammation prostaglandins
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Original Article

6) New Chemical Constituents from the Endophytic Fungus Xylariapapulis Cultivated on Taiwanese Lepidagathisstenophylla

Rec. Nat. Prod. (2016) 10:6 ; 735 - 743
by Yi-Shuan Chen, Hsun-Shuo Chang, Ming-Jen Cheng, Hing -Yuen Chan, Ming -Der Wu, Sung-Yuan Hsieh, Hui-Chun Wang and Ih-Sheng Chen

The endophytic strain Xylaria papulis was originated from the stem of Taiwanese Lepidagathis stenophylla C. B. Clarke ex Hayata (Acanthaceae) growing in the Hengchun Peninsula of southern Taiwan. Bioassay-guided fractionation of an EtOAc -soluble fraction of the liquid fermentate of this strain resulted in the isolation of two new isopimarane-type diterpene glycosides compounds, i.e., xylapapusides A & B (1 & 2), along with five previously identified compounds, 37. The structures of 1 and 2 were elucidated by detailed spectroscopic analysis including 1H- and 13C-NMR, COSY, HSQC, HMBC, and HR-ESI-MS, while the structures of the known compounds 37 were deduced from comparison of their spectral data with those in the literature. The effects of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages were evaluated. Xylapapuside A (1) showed most potent of NO inhibition with E maxvalue of 34.3 μM.

Keywords
Xylaria papulis Lepidagathis stenophylla endophytic fungus isopimarane-type diterpene glycoside
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Original Article

7) Bioassay- guided Isolation of New Urease Inhibitory Constituents from Monotheca buxifolia (Falc.) Fruit and Their Molecular Docking Studies

Rec. Nat. Prod. (2016) 10:6 ; 744 - 749
by Irfan Ullah, Jamshaid Ali Khan, Achyut Adhikari, Ajmal Khan, Peer Abdul Hannan, Abdul Wadood and Umar Farooq

The aim of the study is to explore the inhibitory potential of extract/fractions, and compounds of Monotheca buxifolia fruit against urease enzyme. Crude hydro-ethanolic extract showed a mild inhibitory activity against urease, among fractions ethylacetate fraction was more active followed by n-butanol fraction while there was no inhibitory activity in n-hexane soluble fraction. Ethylacetate fraction was subjected to activity guided isolation yielding four pure compounds, among them two were new i.e. buxifoline-A (1) (First time isolated from natural sources) and buxilide (2) while the other two were first time isolated from the fruit that are isoquercetin (3)and oleanolic acid ­(4). Their structures were elucidated using spectroscopic and spectrometric techniques. Among the isolated compounds compound 3 showed maximum inhibition. In order to understand the binding interactions of the compound 3, it was docked into the active site of urease enzyme. Our study validates the traditional use of the fruit in the treatment of gastritis and urinary tract infections, which is strongly supported by the isolated compound isoquercetin (3).

Keywords
Monotheca buxifolia urease inhibitor isoquercetin bio assay guided isolation buxifoline A buxilide
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Original Article

8) Chemical Composition of the Essential Oil, Total Phenolics, Total Flavonoids and Antioxidant Activity of Methanolic Extracts of Satureja montana L.

Rec. Nat. Prod. (2016) 10:6 ; 750 - 760
by Avni Hajdari, Behxhe Mustafa, Arjeta Kaçiku, Xhavit Mala, Brigitte Lukas, Alban Ibraliu, Gjoshe Stefkov and Johannes Novak

Aerial parts of Satureja montana L. (Lamiaceae) were collected from seven growing wild populations (four populations in Kosovo, two in Albania and one in Montenegro) in 2013 with the aim of assessing the natural variation in the chemical composition of the essential oils, total flavonoids, total phenolics and the antioxidant activity of their methanolic extracts. Essential oils were obtained by steam distillation and analysed using GC-FID and GC-MS, whereas total flavonoids, total phenolics and antioxidant activities were determined using spectrophotometric methods. Sixty-one volatile constituents were identified. The main constituents were myrcene, p-cymene, γ-terpinene, linalool, thymol, carvacrol and viridiflorol. Total phenolics ranged from 68.1 to 102.6 mg/g dry mass, the total flavonoid content ranged from 38.3 to 67.0 mg/g dm, and the antioxidant activity according to the DPPH assay ranged from 253.3 to 342.9 mg TE/g dm and according to the FRAP assay ranged from 8.9 to 11.4 mg TE/g dm. Hierarchical cluster analysis and principal component analyses were used to assess the geographical variations in the essential oil composition. Statistical analysis revealed that the analysed populations are grouped into four main clusters that appear to reflect the environmental impact on the chemical composition, which is influenced by differences in habitat composition, altitude and microclimatic conditions.

Keywords
Winter savoury GC MS volatile oil polyphenols natural variability bioactivity
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Short Report

9) Calotroposide S, New Oxypregnane Oligoglycoside from Calotropis procera Root Bark

Rec. Nat. Prod. (2016) 10:6 ; 761 - 765
by Sabrin R. M. Ibrahim, Gamal A. Mohamed, Lamiaa A. Shaala and Diaa T. A. Youssef

Calotroposide S (1), a new oxypregnane oligoglycoside has been isolated from the n-butanol fraction of Calotropis procera (Ait) R. Br. root bark. The structure of 1 was assigned based on various spectroscopic analyses. Calotroposide S (1) possesses the 12-O-benzoylisolineolon aglycone moiety with eight sugar residues attached to C-3 of the aglycone. It showed potent anti-proliferative activity towards PC-3 prostate cancer, A549 non-small cell lung cancer (NSCLC), and U373 glioblastoma (GBM) cell lines with IC 50 0.18, 0.2, and 0.06 µM, respectively compared with cisplatin and carboplatin.

Keywords
Calotropis procera Asclepiadaceae calotroposide S anti-proliferative activity
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Short Report

10) Antiproliferative Activities of Chemical Constituents Isolated from Thymus praecox subsp. grossheimii (Ronniger) Jalas

Rec. Nat. Prod. (2016) 10:6 ; 766 - 770
by Ramazan Erenler, Ozkan Sen, Ilyas Yildiz and Ali Aydın

Thymus praecox subsp. grossheimii (Ronniger) Jalas is (TPGJ) an aromatic and medicinal plant used as folk medicine and exhibits a variety of biological activities. Aerial part of plant material was boiled in water then extracted with hexane and ethyl acetate sequentially. Flash column chromatography (Sephadex LH-20) and HPLC were used for ethy acetate extract to isolate rosmarinic acid (1), apigenin 7-O-glucoside (2), chrysoeriol (3), apigenin (4), naringenin (5 ), eriodictiol (6), luteolin (7) and globoidnan A (8). The structures of isolated compounds were elucidated by spectroscopic techniques basically 1D, 2D-NMR and LC-TOF/MS/MS. Antiproliferative activity, cytotoxicity of compounds and extract were investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), HT29 (human colon carcinoma) and Vero (African green monkey kidney epithelium) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. Extract and some compounds exhibited significant antiproliferative effects against various cancerous cell lines.

Keywords
Thymus praecox subsp. grossheimii (Ronniger) Jalas antiproliferative activity.
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Short Report

11) Bioactive Natural Products from Piper betle L. Leaves and their α -Glucosidase Inhibitory Potential

Rec. Nat. Prod. (2016) 10:6 ; 771 - 781
by Andreia P. Oliveira, Joana G. Ferreira, Sofia Riboira, Paula B. Andrade and Patrícia Valentão

Piper betle L. can be a valuable proposal for the prevention and treatment of several disorders. In fact, i ts leaves are largely consumed as mouth freshener and masticator, being known for their biological properties. This material possesses several kinds of bioactive natural products. Considering that diabetes is a worldwide disease with strong impact on human health, this work intended to explore the in vitro α-glucosidase inhibitory capacity of P. betle leaves, as well as to improve the knowledge on their metabolic pattern. Thus, a targeted metabolite analysis of the aqueous and ethanolic extract was performed by GC-MS, a similar qualitative profile of both extracts being observed. Fourteen metabolites were determined in P. betle leaves, five of them for the first time. Alanine and β-sitosterol were the main amino acid and sterol, respectively. Stearic and palmitic acids were the predominant fatty acids. A strong capacity to inhibit α-glucosidase was noticed, ethanol extract being more active than the positive control (acarbose) tested under the same conditions. Taking into account the results obtained, this work further extends the potential application of this herbal medicine, suggesting that its consumption may have a positive impact in the prevention and treatment of diabetes disease.

Keywords
Antidiabetic bioactive natural product herbal medicine Piper betle L.
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Short Report

12) Isolation, Identification and Antiproliferative Activity of Triterpenes from the Genus Monotheca A. DC.

Rec. Nat. Prod. (2016) 10:6 ; 782 - 787
by Shabnam Javed, Ikpefan E. Oise, Lutfun Nahar, Fyaz M. D. Ismail, Zaid Mahmood and Satyajit D. Sarker

The Monotheca A. DC. is a monotypic genus of the family Sapotaceae, which is widely distributed in Afghanistan, Djbouti, Northern Somalia, Oman, Pakistan and Southern Ethiopia. North-west Pakistan is the main region where Monotheca buxifolia (Falc.) A. DC., the only species of this genus, locally known as “Gurgura”, grows abundantly. It is an evergreen, fruit-producing medicinal tree. Bioassay-guided fractionation of the aerial parts of M. buxifolia afforded lupeol (1), lupeol acetate (2), betulin (3), oleanolic acid (4) andβ-amyrin (5) from the n-hexane and the chloroform fractions. This is the first report on the isolation, and identification of triterpenes (1-5) as the major compounds in the active fractions with antiproliferative property, and also on the antiproliferative activity of M. buxifolia extract and fractions against the human lung cancer cell line NCI-H460 in vitro.

Keywords
Monotheca buxifolia Sapotaceae antiproliferative property triterpene sulforhodamine B assay lung cancer.
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Short Report

13) LC-MS Based Metabolomics Analysis to Identify Potential Allelochemicals in Wedelia trilobata

Rec. Nat. Prod. (2016) 10:6 ; 788 - 793
by Kamalrul Azlan Azizan, Sarah Ibrahim , Nurul Haizun Abdul Ghani and Mohammad Firdaus Nawawi

Wedelia trilobata is a noxious invasive weed that has been widely cultivated as a decorative and groundcover plant. The plant has been reported to contain diverse bioactive compounds with a broad spectrum of biological activities including allelochemicals. Allelochemicals contribute to allelopathy interactions that suppress the growth and development of nearby plants. Several studies have reported the allelopathic potential of W. trilobata and its negative effects to crop plants. However, relatively little is known about the allelochemicals’ composition and how allelochemicals contribute to the allelopathic behavior of this plant. In order to prove allelopathy, the identification of the causative allelochemicals is required. The identification of potential allelochemicals that serve as biomarkers could be useful for assessing allelopathy interactions. In this study, a liquid chromatography (LC) based metabolomics approach was applied to find biomarkers with allelopathic effects from W. trilobata. Ethanol and water were used to extract metabolites from the leaves of W. trilobata and analyzed using liquid chromatography coupled with quadrupole high-resolution time of flight mass spectrometry (LC-QTOF-MS). Using multivariate statistical analysis (MVA), we identified eight Rt-m/z pairs as candidate marker compounds for assessing allelopathy interactions of W. trilobata. The results highlight the application of metabolomics for understanding of the role of allelochemicals in allelopathy interactions of W. trilobata .

Keywords
Allelopathy metabolomics LC-QTOF-MS Wedelia trilobata
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Short Report

14) Changes of the Anthocyanins and Antioxidant properties of Concord Grape (Vitis labrusca) Pomace After Acid Hydrolysis

Rec. Nat. Prod. (2016) 10:6 ; 794 - 799
by Mustafa Bener, Yixiao Shen, Zhimin Xu and Reşat Apak

Grape pomace contains high levels of valuable antioxidants such as anthocyanins and phenolic compounds that help prevent chronic diseases such as cardiovascular problems and cancers. In this study, Concord grape pomace was soaked in acidic solutions at different time intervals and pHs in a water bath at 80 oC. Five kinds of anthocyanins were released and identified in the pomace after acid hydrolysis. The releasing rate of anthocyanins and antioxidant activities of the acid hydrolyzed pomace extracts were determined by multitest systems. Different antioxidant assays including total antioxidant capacity (TAC), total phenolic content (TPC) and free radical scavenging activity (RSA) were used to evaluate the antioxidant properties of the acid hydrolyzed pomace extracts. The change in antioxidant capacity of the pomace extracts during hydrolysis was correlated with total phenolic content and free radical scavenging activity but had little relationship with anthocyanin contents.

Keywords
Concord grape Vitis labrusca anthocyanin antioxidant phenolic pomace
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Short Report

15) Greek Salvia sclarea L. Essential Oils: Effect of Hydrodistillation Time, Comparison of the Aroma Chemicals Using Hydrodistillation and HS-SPME Techniques

Rec. Nat. Prod. (2016) 10:6 ; 800 - 805
by Aikaterini Koutsaviti, Daphne Irene Tzini and Olga Tzakou

Since the essential oil of Salvia sclarea is used as a flavouring agent, the effect of different extraction techniques (hydrodistillation & HS-SPME) and duration of hydrodistillation (2, 3 and 4 h) with respect to yield, composition and identification rate of extracted essential oils from Greek cultivated S. sclarea aerial blooming parts were investigated. Linalool and linalyl acetate levels seemed to decrease with increasing duration of hydrodistillation, while diterpenes increased dramatically, while the head space analysis showed significantly lower levels of linalool in comparison to its ester. Thus, linalool (5.1-35.8%), linalyl acetate (11.3-37.6%) and sclareol (0.0-41.8%), concerning the oils obtained by hydrodistillation, were the most important metabolites. Solid-phase microextraction yielded mainly oxygenated monoterpenes, especially linalyl acetate (59.3%), followed by cis-linalool oxide (8.6%) and linalool (7.8%).

Keywords
Salvia sclarea solid-phase microextraction linalool linalyl acetate hydrodistillation sclareol.
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Short Report

16) Volatile Constituents of Three Illicium Plants

Rec. Nat. Prod. (2016) 10:6 ; 806 - 811
by Nguyen B. Qinh, Do N. Dai, Bui V. Than, Vo T Dung, Vuong T.T. Hang and Isiaka A. Ogunwande

The chemical composition of volatiles from the leaves of three different Illicium species has been studied. The essential oils were obtained by hydrodistillation and analyzed by GC (FID) and GC-MS. The components by identified by MS libraries and their LRIs. The essential oil contents vary between 0.12% and 0.21% (v/w), calculated on a dry weight basis. Illicium majus J. D. Hooker & Thomson afforded oil whose major constituents were aromadendrene (13.0%), cuparene (8.2%), 1,8-cineole (8.1%) and calamenene (7.8%). However, 1,8-cineole (8.4%), linalool (7.7%), (E)-nerolidol (7.6%) and sabinene (7.1%) were the quantitatively significant compounds of the leaf oil of Illicium micranthun Dunn. The leaf oil of Illicium tsaii A. C. Smith comprised mainly of (E)-nerolidol (15.5%), β-caryophyllene (8.1%), β-cedrene (6.5%), 1,8-cineole (6.3%) and calamenene (6.3%). The chemical composition of the leaf essential oils of these plants from are being reported for the first time.

Keywords
Illicium majus Illicium micranthum Illicium tsaii monoterpenes sesquiterpenes
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Short Report

17) Antioxidant and Hepatoprotective Activities of Flavonoids from Bauhinia hookeri

Rec. Nat. Prod. (2016) 10:6 ; 812 - 817
by Eman Al-Sayed, Mai F. Tolba and Maarit Karonen

In a previous study, the total ethanol extract of Bauhinia hookeri  showed a significant hepatoprotective effect in CCl 4-induced toxicity model in mice. However, the active components responsible for the activity were not identified. Therefore, this study was undertaken to determine if the activity of B. hookeri extract is due to its flavonoid content. The hepatoprotective activity of B. hookeri flavonoids was determined by measuring the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the culture medium of HepG2 cells challenged with CCl 4. The lipid peroxidation and antioxidant parameters, superoxide dismutase (SOD) and glutathione (GSH) were estimated in the cell lysates. The isolated flavonoids were identified by mass, UV and NMR spectral data. This study revealed that B. hookeri flavonoid fraction and its pure compounds ( kaempferol 3-O-β- D-glucoside, quercetin 3-O-β- D-glucoside and c atechin 3-O-α- L-rhamnoside) possess a promising hepatoprotective activity as evidenced from the normalized levels of ALT and AST. This was attributed partly to their potent antioxidant activity as demonstrated by the increased GSH levels, SOD activity and reduced lipid peroxidation. The whole flavonoid fraction showed the highest cytoprotective activity and was more effective than silymarin. This study highlights a promising natural hepatoprotective remedy derived from B. hookeri.

Keywords
Antioxidant Bauhinia hookeri cytoprotection flavonoids lipid peroxidation oxidative stress
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Short Report

18) Essential Oil Composition and Antibacterial Activity of Tanacetum hololeucum from Iran

Rec. Nat. Prod. (2016) 10:6 ; 818 - 823
by Hamed Shamkhani, Naser Nasiri, Atousa Aliahmadi and Ali Sonboli

Composition and antibacterial activity of the hydro-distilled essential oil from aerial parts of Tanacetum hololeucum from Iran were investigated. The oil of T. hololeucum was analyzed by GC-FID and GC-MS. The major compounds were found to be artemisia alcohol (22.8%), yomogi alcohol (19.4%), artemisyl acetate (12.9%), γ-eudesmol (12.1%) and camphor (10.5%). The antibacterial activity of the oil was tested against four bacteria (Staphylococcus aureus, Bacillus cereus, KlebsiellaPneumoniae and Escherichia coli) by disk diffusion and determination of MIC and MBC values. The results showed that Gram-positive bacteria were highly inhibited by the oil with MIC values of 2 mg/mL. The most resistant strain was KlebsiellaPneumoniae with the highest MIC and MBC values of 16 and >32 mg/mL, respectively. Considering sensitivity screening it is notable that the antibacterial property of the oil from T. hololeucum could be attributed mainly to the synergistic property of main compounds.

Keywords
Tanacetum hydro-distillation yomogi alcohol artemisia alcohol Iran
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